ANRF-SERB-Sponsored
NATIONAL CRYO-EM FACILITY
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High resolution structure determination of proteins and protein complexes
High resolution structure determination of proteins and protein complexes
State-of-the-art cryo-EM instruments for data collection and atomic resolution structure determination of biochemically purified single particles
Sample Preparation
Glow discharge: Hydrophilization of TEM carbon support films
Vitrobot: Automated device for vitrification (plunge freezing) of aqueous samples
Data Collection and Structure Determination
Titan Krios G4: 80-300kV, FEG. 12-grid liquid nitrogen-cooled stage, Gatan Quantum K3 Energy Filter (GIF) with a Gatan K3 direct electron detector
Structure solution: Determination of high-resolution structures of specimens
Questions?
Questions?
Contact: cryo_em@iitk.ac.in
Phone: 0512-6792513
Acknowledgements
Acknowledgements
Location
Location
Publications:
Molecular mechanism of distinct chemokine engagement and functional divergence of the human Duffy antigen receptor. Saha S#, Khanppnavar B#, Maharana J#, Kim J, Carino CMC, Daly C, Houston S, Sharma S, Zaidi N, Dalal A, Mishra S, Ganguly M, Tiwari D, Kumari P, Jhingan GD, Yadav PN, Plouffe B, Inoue A, Chung KY, Banerjee R*, Korkhov VM* and Shukla AK*. Cell, 2024 (in press)
Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution. Yadav M#, Sarma P#, Maharana J, Ganguly M, Mishra S, Zaidi N, Dalal A, Singh V, Saha S, Mahajan G, Sharma S, Chami M, Banerjee R* and Shukla AK*. Nature Communications, 2024 Mar 2;15(1):1939.
Molecular insights into atypical modes of β-arrestin interaction with seven transmembrane receptors. Maharana J#, Sano F#, Sarma P#, Yadav M, Longhan D, Stepniewski TM, Chaturvedi M, Ranjan A, Singh V, Saha S, Mahajan G, Chami M, Shihoya W, Selent J, Chung KY, Banerjee R*, Nureki O* and Shukla AK*. Science, 2024 Jan 5;383(6678):101-108.
Molecular basis of anaphylatoxin recognition, activation, and signaling-bias at complement receptors. Yadav MK#, Maharana J#, Yadav R#, Saha S#, Sarma P#, Soni S, Singh V, Saha S, Ganguly M, Li XX, Mahapatra S, Mishra S, Khant HA, Chami M, Woodruff TM, Banerjee R*, Shukla AK* and Gati C*. Cell, 2023, Oct 26;186(22):4956-4973.e21.
Structural snapshots uncover a key phosphorylation motif in GPCRs driving β-arrestin activation. Maharana J#, Sarma P#, Yadav MK, Saha S, Singh V, Saha S, Chami M, Banerjee R* and Shukla AK*. Molecular Cell, 2023 Jun 15;83(12):2091-2107.e7.
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